The ChIP assay is a widely used technique that enriches DNA fragments to which a specific protein of interest or histone modification is bound. With the increasing performance of sequencing platforms, ChIP-seq is the leading technology for genome-scale analysis of protein-DNA interactions. The latest version of the popular Chromatrap ChIP-Seq kit enables ChIP to be performed from as little as 50mg of a wide range of extracted tissue samples. The new protocol incorporates optimised lysis, hyptonic buffers and guidelines on how to achieve maximum extraction of chromatin from limited sample quantities. This ground-breaking performance, that delivers great results whilst saving your precious samples, is demonstrated in a number of recent research publications. |